Characterization of chemical profiles in Cannabis sativa: evaluation of a popular extraction method to identify chemotypes
DOI:
https://doi.org/10.56152/StevianaFacenV15N2A3_2024Keywords:
Cannabis sativa, Tetrahydrocannabinol (THC), Cannabidiol (CBD), High-Performance Liquid Chromatography (HPLC), Molecular markers, ChemotypesAbstract
This study addresses the characterization of cannabinoids in different Cannabis sativa varieties cultivated in Paraguay, focusing on determining the ratio between the primary phytocannabinoids, tetrahydrocannabinol (THC) and cannabidiol (CBD). Using advanced high-performance liquid chromatography (HPLC) techniques, cannabis extracts were analyzed, identifying significant variations in cannabinoid concentrations that highlight the intrinsic chemical diversity
among the studied varieties. Additionally, homemade extraction methods were evaluated, using ethanol and cold maceration, followed by low-temperature solvent evaporation. The results showed variable CBD ratios, indicating the suitability of the extracts for therapeutic and industrial applications (hemp or non-psychoactive cannabis). The research was conducted considering national regulations, ensuring the integrity and applicability of the findings. This work not only contributes to the existing scientific corpus on cannabis but also emphasizes the importance of research aimed at optimizing its
cultivation and medicinal use, aligned with national interests expressed in Decree No. 3999.
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