Inhibitory ethanolic extract of Plantago major L. inhibitory effect on radicular meristematic growth of Allium cepa L.
DOI:
https://doi.org/10.56152/StevianaFacenV14N2A2_2022Keywords:
productos naturales, citotoxicología, meristemaAbstract
The use of natural products, such as plantain (Plantago major) extract, has generated an increase in the commercialization of substances, which in some cases are not studied for their possible toxic effects. The effect of the ethanolic extract of plantain (P. major) on root growth was studied using the standardized technique of crushing root meristematic cells of Allium cepa. Knop® commercial ethanolic extract (30 mg/mL concentration) was used. A root growth system was established under controlled conditions of temperature, oxygenation and darkness. Three groups were established (Negative Control, Experimental Group with increasing time and Group with increasing dose of 0.75 mg/mL, 1.5 mg/mL, 7.5 mg/mL, 15 mg/mL and 30 mg/mL). The optimal dose for the study was evaluated beforehand. Eighteen Allium cepa bulbs were used, with 3 bulbs for each study group. The exposure to the ethanolic extract was at 24, 48, 72, 96 and 120 h. Crushing and staining with acetochlorohydric orcein were performed. A thousand cells per plate were observed and counted. The values obtained were analyzed through of mean comparison and ANOVA test. The results were discussed and analyzed in terms of the effect of P.major on cell cycle variations and mitotic index. The results obtained indicate that the ethanolic extract of P. major at concentrations higher than 7.5 mg/ mL are cytotoxic to root meristems; and that after 48 h of exposure to the extract, cell proliferation is drastically reduced.
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Adom M.B., Taher M, Mutalabisin M.F., Amri M.S., Abdul Kudos M.B., Wan Sulaiman M.W.A., Sengupta P & Susanti, D. (2017). Chemical constituents and medical benefits of Plantago major. Biomed Pharmacother. Dec, 96,348-360.
Albuquerque, U.P. y Hurrell, J.A. (2010). Ethnobotany: one concept and many interpretations. In Albuquerque UP, Hanazaki N: Recent developments and case studies in Ethnobotany. UPEEA, Recife, Brasil.
Alvarado-Villanueva, V. y Moromi Nakata, H. (2010). Plantas medicinales: efecto antibacteriano in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense y Camellia sinensis sobre bacterias de importancia estomatológica. Odontol Sanmarquina, 13(2) ,21-5.
Alfredo, P.P., Anaruma, C.A., Pião, A.C., João, S.M. y Casarotto, R.A. (2009). Effects of phonophoresis with Arnica montana onto acute inflammatory process in rat skeletal muscles: An experimental study. Ultrasonics,49(4-5), 466-471.
Bell D.R., Gochenaur, K. (2006). Direct vasoactive and vasoprotective properties of anthocyanin-rich extracts. J Appl Physiol, 100(4),1164-70.
Bye, R. (2003). Plantas popularmente utilizadas para afecciones del aparato digestivo, diarrea y parásitos en México. Bioactive Agents from Dryland Biodiversity of Latin America.
Cabrera A., Mach, N. (2012). Flavonoides como agentes quimiopreventivos y terapéuticos contra el cáncer de pulmón. Rev Esp. Nutr. Hum. Diet, 17(2), 91–92.
Calderón Pongo, B. (2018). Evaluación del extracto etanólico del Plantago major y nistatina para inhibir el crecimiento in vitro de Candida albicans. Juliaca.
Fiestas, I. y Huanca, F. (2018). Extracto hidroalcohólico de Plantago major L. y su efecto antibacteriano sobre cultivos de Streptococcus pyogenes estudios in vitro. [Tesis. Facultad de Ciencias Farmacéuticas y Bioquímica, Universidad Inca Garcilaso de la Vega, Lima. Perú]
Fiskesjó, G. (1985). The Allium test as a standard in environmental monitoring. Hereditas, 102, 99-112.
Fiskesjö, G. & Dutka, B. (1996). Toxicological monitoring of drinking waters in developing countries. Proceedings of WaterTox [Workshop]. Ottawa: Burlington National Water Research Institute.
Garro Monje, G., Jimenes, Q. K., Castro, P. S. y Montero, C. V. (2021). Optimización del protocolo de establecimiento de cultivos celulares de Plantago major (llantén) para la comprobación de la actividad cicatrizante de un producto farmacéutico y determinación efecto biológico contra la infección de H. pylori en modelos in vitro. [Proyecto de Investigación (Código: 1510044). Instituto Tecnológico de Costa Rica, Vicerrectoría de Investigación y Extensión, Dirección de Investigación].
Gálvez, M., López-Lázaro, M., Navarro, E., Mederos, S., Martín-Cordero, C. y Ayuso, M.J. (2004). Citotoxicidad de Plantago major L. Canarias Médica y Quirúrgica I, 2(4).
Golberg, D. (2015). La importancia actual de las hierbas medicinales. Agroconsultora Plus. http://www.agroconsultoraplus.com/curso-fitoterapia.
Gutiérrez, C. (2014). Cuantificación de alteraciones cromosómicas en meristemos radiculares de Allium cepa expuestos a extractos etanólico y acuoso de Plantago major. [Tesis de Grado. Universidad Nacional de Trujillo. Facultad de Ciencias Biológicas].
Hernández M.P., Civitella S.M., y Rosato, V.G. (2010). Uso medicinal popular de plantas y líquenes de la Isla Paulino, Provincia de Buenos Aires, Argentina. Bol Latinoam Caribe Plant. Med. Aromat. 9, 258–268.
Hiltibran, R. C., Wadkins, C. L., & Nicholas, H. J. (1953). The distribution of triterpenes in rugel's plantain1. Journal of the American Chemical Society, 75(20), 5125-5126.
Hurrell, J.A. y Albuquerque, U.P. (2012). Is ethnobotany an ecological science? Steps towards a complex. Ethnobot. Ethnobiol. Conserv.
IBM Corp. Released (2015). IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp.
INBio. (1997). Jerarquía taxonómica. http://www.inbio.ac.cr/bims/k03/p13/c045/o0139/f01349/g008585/s027112.htm.
Jiménez, J. (2012). Péptidos bioactivos de la leche. Ponencia presentada en el 1er Congreso Nacional Producción, Calidad, Transformación, Comercialización y Nutrición de Leche y Derivados. Acapulco-México.
Kaewpiboon, C., Lirdprapamongkol, K., Srisomsap, C., Winayanuwattikun, P., Yongvanich, T., Puwaprisirisan, P., Svasti, J. & Assavalapsakul, W. (2012). Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants. BMC Complement and Alternat Med 12: 217.
Kawashty, S. A., Abdalla, M. F., & Saleh, N. A. M. (1994). Flavonoids of Plantago species in Egypt. Biochemical Systematics and Ecology, 22(7), 729-733.
Le Bail, C., Varnat, F., Nicolas, J. C. & Habrioux, G. (1998). Estrogenic and antiproliferative activities on MC F-7 human breast cancer cells bv flavonoids. Cancer Lett. 130, 209-216.
Luz, A.C., Pretti, I.R., Dutra, J.C.V., Batitucci, M.C.P. (2012). Avaliação do potencial citotóxico e genotóxico de Plantago major L. em sistemas teste in vivo. Rev. Bras. Pl. Med., Botucatu, 14(4), 635-642.
Motoo, Y. & Sawabu, N. (1994). Antitumor effects of saikosaponins, baicalin and baicaleina on human hepatoma cell lines. Cancer Lettera, 86, 91-95.
Nishibe, S., Tamayama, Y., Sasahara, M., & Andary, C. (1995). A phenylethanoid glycoside from Plantago asiatica. Phytochemistry, 38(3), 741-743.
Obregón, L. (2001). Fitoterapia: perspectivas de la fitoterapia para el nuevo milenio. [Ponencia]. Primer Congreso Internacional de Plantas Medicinales y Aromáticas. Cali, Colombia.
Ozaslam, M., Karagoz, D., Kilic, H., Sari, I. & Karagoz, A. (2007). In vivo antitumoral effect of Plantago major L. extract on Balb/C Mouse with Erlich Tumor”. The American Journal of Chinese Medicine, 35(5), 841-851
Pailer M., Haschke-Hofmeister E. (1969). Contents from Plantago major. Planta Med, 17 (2), 139–145.
Post, F.M., Varma, R.S. (1992). Growth inhibitory effects of bioflavonoids and related compounds on human leukemic CEM-C1 and CEM-C7 cells. Cancer Lett., 67, 207-213.
Pensantes-Sangay, S. J., Calla-Poma, R. D., Requena-Mendizabal, M. F., Alvino-Vales, M. I., & Millones-Gómez, P. A. (2020). Chemical composition and antibacterial effect of Plantago major extract on periodontal pathogens. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 20, e0012.
Ringbom, T., Segura, L., Noreen, Y., Perera, P., & Bohlin, L. (1998). Ursolic acid from Plantago major, a selective inhibitor of cyclooxygenase-2 catalyzed prostaglandin biosynthesis. Journal of natural products, 61(10), 1212-1215.
Ryu, S.Y., Choi, S.U., Lee, C.A., Lee, S.H., Ahn, J.W., & Zee, O. P. (1994). Antitumor activity of some phenolic components in plants. Arch. PharmacoI. Res., 17, 42-44.
Sabag, V.; Pinto, J.; Zabalaga, S. y Camacho, M. (2010). Formulación de un fitomedicamento con actividad gastroprotectora a partir de extractos de llantén (Plantago major). BIOFARBO, 18 (2), 44-52.
Santiago Pérez, M. I., Hervada Vidal, X., Naveira Barbeito, G., Silva, L. C., Fariñas, H., Vázquez, E., ... y Mújica, O. J. (2010). El programa epidat: usos y perspectivas. Revista Panamericana de Salud Pública, 27, 80-82
Sanz, M. J., Ferrandiz, M. L., Cejudo, M., Terencio, M. C., Gil, B., Bustos, G., ... & Alcaraz, M. J. (1994). Influence of a series of natural flavonoids on free radical generating systems and oxidative stress. Xenobiotica, 24(7), 689-699.
Schneider G. (2009). Arzneidrogen, ein kompendium für pharmazeuten, biologen und chemiker. Wissenschaftsverlag, Mannheim, Germany.
Yuting, C., Rongliang, Z., Zhongjian, J., & Yong, J. (1990). Flavonoids as superoxide scavengers and antioxidants. Free Radical Biology and Medicine, 9(1), 19-21.
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