Molecular detection of Anaplasma platys from canine capillary blood dog filter paper
Keywords:
Anaplasma platys, hemopathogen, filter paper, cPCR, dogAbstract
Due to the high prevalence of Anaplasma platys in tropical areas, the present study evaluated the usefulness of filter paper impregnated with canine capillary blood as an alternative method for the molecular detection of A. platys. A total of 101 genomic DNA samples extracted from dried blood impregnated on modified filter paper were analyzed. DNA extraction was performed using ion-exchange resins (Chelex 100®), and a fragment of the p44 gene was amplified by conventional PCR (cPCR). Amplification products were visualized by 1.5% agarose gel electrophoresis. The results showed that 9 out of 101 samples (8.91%) tested positive for A. platys, demonstrating the presence of the hemopathogen in the analyzed canine population. The study findings support the use of filter paper impregnated with capillary blood as a reliable and efficient alternative for the collection, transport, and storage of blood samples in the molecular diagnosis of A. platys, thus contributing to epidemiological surveillance and clinical management of this infection in canine populations.
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Copyright (c) 2026 Alba María Araujo Oviedo, Selva Lucía Amarilla, Jeanette Filippi Dallmann, María de Lourdes Quiñónez Narváez, Lino César Ramírez Ayala, Guillermo Giménez Bareiro, Ingrid Paloma Florentín Ibarra, María Inés Rodríguez Acosta, Olga Lorena Núñez Yegros
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