Análisis filogenético y de presión evolutiva de secuencias nucleotídicas del gen VP4 de especies de enterovirus humanos

Authors

  • Alina Acosta Cabello
  • Graciela Russomando
  • Emilio E. Espínola

Keywords:

Enterovirus humanos, secuencias nucleotídicas, gen VP4, análisis filogenético, presión selectiva

Abstract

El género Enterovirus es un grupo viral que afecta a un amplio rango de hospederos, entre ellos los humanos (especies A, B, C, y D), causan enfermedades respiratorias, gastrointestinales, neurológicas, y otras, y son altamente contagiosos. Los síntomas pueden ser leves o graves. El objetivo del trabajo fue analizar la variación nucleotídica, filogenética y de presión evolutiva de secuencias nucleotídicas del gen VP4 de las cuatro especies que afectan a los humanos. Se emplearon 92 secuencias nucleotídicas disponibles en la base de datos GenBank; éstas se editaron con el software BioEdit y se alinearon con Clustal W; las relaciones filogenéticas se determinaron con MEGA6, y las presiones evolutivas con los algoritmos SNAP y SLAC. Se encontró que la identidad nucleotídica mínima intra-especie fue de 43,2% (especie B) a 72,6% (especie D). Los genotipos más variables por especie fueron EV-71 (A), Echovirus 2 (B), EV-118 (C), y EV-94 (D). El análisis de presión evolutiva mostró que el gen VP4 en las cuatro especies evoluciona bajo presión selectiva negativa. Esto indicaría que la alta tasa mutacional y eventos de recombinación no tienen un rol significativo en la evolución de este gen, debido probablemente a la localización interna de la proteína VP4.

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Published

2016-08-01

How to Cite

Acosta Cabello, A., Russomando, G., & Espínola, E. E. (2016). Análisis filogenético y de presión evolutiva de secuencias nucleotídicas del gen VP4 de especies de enterovirus humanos. Memorias Del Instituto De Investigaciones En Ciencias De La Salud, 14(2). Retrieved from https://revistascientificas.una.py/index.php/RIIC/article/view/1854

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