Erratum to “Glaesserella parasuis: serotypes and virulence of strains isolated from pigs in Itapúa-Paraguay between February 2022 and March 2023”

Abstract

The authors sincerely apologize for the unintentional typographical error on page 87, Materials and Methods section.
The full content of that section should read as follows the inclusion is displayed in bold:
Molecular Typing and Virulence. The primers described by Howell (2015), with modifications made by Lacouture et al. (2017), were used to perform molecular typing. Three primer mixtures were used as follows: PM1: funB (Ser1), glyC (Ser3), wciP (Ser4), funQ (Ser7), funAB (Ser14); PM2: wzx (Ser2), funV (Ser9), gltP (Ser13), funI (Ser15) and PM3: wcwK (Ser5/12), gltI (Ser6), scdA (Ser8), funX (Ser10), amtA (Ser11). Each PCR reaction consisted of 12.5 μL of 2x GoTaq® G2 Colorless Master Mix (Promega); 1 mM of each of the primers, csp of nuclease-free water and 2 μL of extracted DNA, with a final volume of 25 µL. The cycling conditions were the same as described by Lacouture et al., (2017). To identify the presence of virulence-related genes, the methodology described by Galofré-Mila et al. (2017) for the amplification of vtaA virulence genes was used.

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