Glaesserella parasuis: serotypes and virulence of strains isolated from pigs in Itapúa-Paraguay between February 2022 and March 2023
DOI:
https://doi.org/10.57201/IEUNA2313373Keywords:
Glässer's disease, multiplex PCR, molecular serotypingAbstract
Glaesserella parasuis is usually found in the respiratory tract of pigs, although there are strains associated with severe systemic diseases, causing economic losses worldwide. There are 15 characterized serotypes associated with different degrees of virulence. In Paraguay, the circulating serotypes are unknown. The objective of this work was to identify G. parasuis serotypes present in the Department of Itapúa. A total of 62 pigs with respiratory symptoms from five pig production farms in Itapúa were included during the month of February 2022 to March 2023. The isolates were made from lung and turbinate samples collected at necropsies and nasal swabs from live animals. The samples were cultured in Chocolate Agar + NAD, after 48 hours of incubation at 37°C, biochemical tests were performed on the suspected colonies, then DNA extraction was performed on the confirmed ones, followed by multiplex PCR using specific pair of primers for each serotype and species. In all farms, the presence of G. parasuis was identified, on a total of 12 strains, including Serotype 2 (1), Serotype 3 (3), Serotype 7 (1), Serotype 8 (1) and others Non-typeable (6), identifying the presence of the VtaA virulence gene in all the isolated strains, except the one corresponding to SV8. Thus, this work becomes the first report of G. parasuis serotypes in Itapúa-Paraguay
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References
Aragon, V., Segalés, J. & Tucker, A. W. (2019). Glässer’s Disease. In Diseases of swine (pp. 844–853).
Cerdà-Cuéllar, M. & Aragon, V. (2008). Serum-resistance in Haemophilus parasuis is associated with systemic disease in swine. Veterinary Journal, 175(3), 384–389. https://doi.org/10.1016/j.tvjl.2007.01.016
Costa-Hurtado, M., Barba-Vidal, E., Maldonado, J., & Aragon, V. (2020). Update on Glässer’s disease: How to control the disease under restrictive use of antimicrobials. In Veterinary Microbiology (Vol. 242). Elsevier B.V. https://doi.org/10.1016/j.vetmic.2020.108595
Frandoloso, R., & Resende de Macebo, N. (2022). Doença de Glässer. In R. M. C. Guedes & D. Barcellos (Eds.), Doenças dos Suínos (Tercera, pp. 196–207).
Galofré-Milà, N., Correa-Fiz, F., Lacouture, S., Gottschalk, M., Strutzberg-Minder, K., Bensaid, A., Pina-Pedrero, S., & Aragon, V. (2017). A robust PCR for the differentiation of potential virulent strains of Haemophilus parasuis. BMC Veterinary Research, 13(1). https://doi.org/10.1186/s12917-017-1041-4
Howell, K. J., Peters, S. E., Wang, J., Hernandez-Garcia, J., Weinert, L. A., Luan, S. L., Chaudhuri, R. R., Angen, Aragon, V., Williamson, S. M., Parkhill, J., Langford, P. R., Rycroft, A.N., Wren, B. W., Maskell, D. J., Tucker, A. W. & Consortium, D. J. (2015). Development of a multiplex
PCR assay for rapid molecular serotyping of Haemophilus parasuis. Journal of clinical microbiology, 53(12), 3812-3821.
Lacouture, S., Rodriguez, E., Strutzberg-Minder, K. & Gottschalk, M. (2017). Canada: Serotyping of Haemophilus parasuis field isolates from disease pigs in Quebec by indirect hemagglutination assay and multiplex polymerase chain reaction (PCR). The Canadian Veterinary Journal, 58(8), 802.
Olvera, A., Pina, S., Macedo, N., Oliveira, S., Aragon, V. & Bensaid, A. (2012). Identification of potentially virulent strains of Haemophilus parasuis using a multiplex PCR for virulence-associated autotransporters (vtaA). Veterinary Journal, 191(2), 213–218. https://doi.org/10.1016/j.tvjl.2010.12.014
Pires Espíndola, J., Balbinott, N., Trevisan Gressler, L., Machado, G., Silene Klein, C., Rebelatto, R., Gutiérrez Martín, C. B., Kreutz, L. C.,
Schryvers, A. B. & Frandoloso, R. (2019). Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil. PeerJ, 7, e6817. https://doi.org/10.7717/peerj.6817
Schuwerk, L., Hoeltig, D., Waldmann, K. H., Strutzberg-Minder, K., Valentin-Weigand, P. & Rohde, J. (2020). Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods. Veterinary Research, 51(1). https://doi.org/10.1186/s13567-020-00862-1
Thermoscientific. (2021). GeneJET Viral DNA RNA Purification User Guide.
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Copyright (c) 2023 Nadia Denisse Zaracho Paniagua, Yasmine Maluff Ladan, Liliana Noelia Talavera Stefani, Yanina Dionisia Sapper Lacy, Carolina Elizabeth Prendeski Stolaruk, Eliane Aya Nishii Encina
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