Metabolitos secretados por aislados nativos de Trichoderma spp. y su actividad antifúngica frente a Rhizoctonia solani y Sclerotinia sclerotiorum, patógenos del pimientoos del pimiento

Autores/as

  • Dani Daniel Ruiz Díaz Mendoza Universidad Nacional de Asunción, Facultad de Ciencias Químicas, Departamento de Química Biológica, San Lorenzo, Paraguay https://orcid.org/0000-0001-9821-5656
  • Romina Nathalia Lezcano Escobar Universidad Nacional de Asunción, Facultad de Ciencias Químicas, Departamento de Química Biológica, San Lorenzo, Paraguay
  • Alberto Anastacio Cubilla-Rios Universidad Nacional de Asunción, Facultad de Ciencias Químicas, Departamento de Química Biológica, San Lorenzo, Paraguay https://orcid.org/0000-0003-3357-9197
  • María Cristina Romero-Rodríguez Universidad Nacional de Asunción, Facultad de Ciencias Químicas, Departamento de Química Biológica, San Lorenzo, Paraguay https://orcid.org/0000-0003-3979-0348
  • María Eugenia Flores-Giubi Universidad Nacional de Asunción, Facultad de Ciencias Químicas, Departamento de Química Biológica, San Lorenzo, Paraguay https://orcid.org/0000-0002-1572-9983
  • Javier E. Barúa Chamorro Facultad de Ciencias Químicas, Universidad Nacional de Asunción https://orcid.org/0000-0002-8164-3432

Palabras clave:

control biológico, fitopatógenos, antibiosis, productos naturales, micotoxinas

Resumen

El pimiento (Capsicum spp.), cultivo hortícola de amplia distribución y fácil manejo que representa una fuente clave de ingresos para pequeños y medianos productores, ve amenazada su producción por fitopatógenos como Rhizoctonia solani y Sclerotinia sclerotiorum, responsables de la pudrición de raíz y la podredumbre blanca, respectivamente. Trichoderma spp., hongos ampliamente reconocidos por su capacidad antagonista, se utilizan eficazmente como agentes de control biológico debido a mecanismos como la competencia, la antibiosis y el micoparasitismo. El objetivo del estudio fue evaluar la producción de metabolitos por aislados nativos de Trichoderma spp. y determinar su actividad antifúngica frente a R. solani y S. sclerotiorum.  Todos los extractos orgánicos evaluados mostraron algún grado de actividad antifúngica. En particular, el extracto orgánico del aislado Trichoderma sp. FCQ14 inhibió completamente el crecimiento de ambos patógenos, mientras que el extracto de T. asperellum FCQ42 logró una inhibición del 100 % frente a S. sclerotiorum. En el extracto de Trichoderma sp. FCQ14, se identificó un compuesto mayoritario cuyas propiedades espectroscópicas coinciden con las de trichodermina, un trichoteceno conocido por inhibir la síntesis proteica en eucariotas. Se sugiere que este metabolito es responsable de la actividad antifúngica observada.

Descargas

Los datos de descarga aún no están disponibles.

Referencias

Ahluwalia, V., Kumar, J., Rana, V. S., Sati, O. P., & Walia, S. (2015). Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity. Natural Product Research, 29 (10), 914–920. https://doi.org/10.1080/14786419.2014.958739

Barúa, J. E., De la Cruz, M., De Pedro, N., Cautain, B., Hermosa, R., Cardoza, R. E., Gutiérrez, S., Monte, E., Vicente, F., & Collado, I. G. (2019). Synthesis of trichodermin derivatives and their antimicrobial and cytotoxic activities. Molecules, 24(20), 3811. https://doi.org/10.3390/MOLECULES24203811

Chen, H. L., Lo, Y. H., Lin, C. L., Lee, T. H., Leung, W., Wang, S. W., Lin, I. P., Lin, M. Y., & Lee, C. H. (2022). Trichodermin inhibits the growth of oral cancer through apoptosis-induced mitochondrial dysfunction and HDAC-2-mediated signaling. Biomedicine & Pharmacotherapy, 153, 113351. https://doi.org/10.1016/J.BIOPHA.2022.113351

Chen, J., Ullah, C., Reichelt, M., Beran, F., Yang, Z. L., Gershenzon, J., Hammerbacher, A., & Vassão, D. G. (2020). The phytopathogenic fungus Sclerotinia sclerotiorum detoxifies plant glucosinolate hydrolysis products via an isothiocyanate hydrolase. Nature Communications, 11(1). https://doi.org/10.1038/S41467-020-16921-2

Chen, J., Ullah, C., Reichelt, M., Gershenzon, J., & Hammerbacher, A. (2019). Sclerotinia sclerotiorum circumvents flavonoid defenses by catabolizing flavonol glycosides and aglycones. Plant Physiology, 180(4), 1975–1987. https://doi.org/10.1104/PP.19.00461

Cheng, X., Dai, T., Hu, Z., Cui, T., Wang, W., Han, P., Hu, M., Hao, J., Liu, P., & Liu, X. (2022). Cytochrome P450 and glutathione S-transferase confer metabolic resistance to SYP-14288 and multi-drug resistance in Rhizoctonia solani. Frontiers in Microbiology, 13, 806339. https://doi.org/10.3389/FMICB.2022.806339

Cortez-Lázaro, A. A., Vázquez-Medina, P. J., Caro-Degollar, E. M., García Evangelista, J. V., Cortez-Lázaro, R. A., Rojas-Paz, J. L., Legua-Cardenas, J. A., Fernandez-Herrera, F., Pesantes-Rojas, C. R., Ocrospoma-Dueñas, R. W., Oliva-Cruz, S. M., Manes-Cangana, G. A., Romero Bozzetta, J. L., & Leiva Espinoza, S. T. (2025). Global trends in Trichoderma secondary metabolites in sustainable agricultural bioprotection. Frontiers in Microbiology, 16, 1595946. https://doi.org/10.3389/FMICB.2025.1595946

Cubilla-Ríos, A. A., Ruíz-Díaz-Mendoza, D. D., Romero-Rodríguez, M. C., Flores-Giubi, M. E., & Barúa-Chamorro, J. E. (2019). Antibiosis of proteins and metabolites of three species of Trichoderma against paraguayan isolates of Macrophomina phaseolina. Agronomía Mesoamericana, 30(1), 63–77. https://doi.org/10.15517/AM.V30I1.34423

El-Kazzaz, M. K., Ghoneim, K. E., Agha, M. K. M., Helmy, A., Behiry, S. I., Abdelkhalek, A., Saleem, M. H., Al-Askar, A. A., Arishi, A. A., & Elsharkawy, M. M. (2022). Suppression of pepper root rot and wilt diseases caused by Rhizoctonia solani and Fusarium oxysporum. Life, 12(4). https://doi.org/10.3390/LIFE12040587

Erper, I., Özer, G., Zholdoshbekova, S., Yildirim, E., & Turkkan, M. (2021). First report of Rhizoctonia solani AG 4 HG-III causing root rot of pepper in Kyrgyzstan. Journal of Plant Pathology, 103(1), 359. https://doi.org/10.1007/S42161-020-00681-5

Frimpong, G. K., Adekunle, A. A., Ogundipe, O. T., Solanki, M. K., Sadhasivam, S., & Sionov, E. (2019). Identification and toxigenic potential of fungi isolated from Capsicum peppers. Microorganisms, 7(9), 303. https://doi.org/10.3390/MICROORGANISMS7090303

Gao, Y., Miles, S. L., Dasgupta, P., Rankin, G. O., Cutler, S., & Chen, Y. C. (2021). Trichodermin induces G0/G1 cell cycle arrest by inhibiting C-MYC in ovarian cancer cells and tumor xenograft-bearing mice. International Journal of Molecular Sciences, 22(9), 5022. https://doi.org/10.3390/IJMS22095022

Gibbs, J. N. (1967). A Study of the Epiphytic Growth Habit of Fomes annosus. Annals of Botany, 31(4), 755–774. https://doi.org/10.1093/OXFORDJOURNALS.AOB.A084180

Guzmán-Guzmán, P., Etesami, H., & Santoyo, G. (2025). Trichoderma: a multifunctional agent in plant health and microbiome interactions. BMC Microbiology 2025 25, 1, 25(1), 1–17. https://doi.org/10.1186/S12866-025-04158-2

Hewedy, O. A., Abdel-Lateif, K. S., & Bakr, R. A. (2020). Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper. Journal of Basic Microbiology, 60(2), 126–135. https://doi.org/10.1002/JOBM.201900493

Hewedy, O. A., Abdel Lateif, K. S., Seleiman, M. F., Shami, A., Albarakaty, F. M., & El-Meihy, R. M. (2020). Phylogenetic Diversity of Trichoderma strains and their antagonistic potential against soil-borne pathogens under stress conditions. Biologyv 2020. 9 (8), 189. https://doi.org/10.3390/BIOLOGY9080189

Jambhulkar, P. P., Singh, B., Raja, M., Ismaiel, A., Lakshman, D. K., Tomar, M., & Sharma, P. (2024). Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India. Scientific Reports, 14(1). https://doi.org/10.1038/S41598-024-58302-5

Jiménez-Pérez, O., Gallegos-Morales, G., Hernández-Castillo, F. D., Cepeda-Siller, M., Espinoza-Ahumada, C. A. (2022). Characterization and pathogenicity of a Pythium aphanidermatum isolate causing ‘damping off’ in pepper seedlings. Revista Mexicana de Fitopatología, 40(1), 116–130. https://doi.org/10.18781/R.MEX.FIT.2109-3

Joo, J. H., & Hussein, K. A. (2022). Biological control and plant growth promotion properties of volatile organic compound-producing antagonistic Trichoderma spp. Frontiers in Plant Science, 13, 897668. DOI: 10.3389/fpls.2022.897668

Katsumata, S., Toshima, H., & Hasegawa, M. (2018). Xylosylated detoxification of the rice flavonoid phytoalexin sakuranetin by the rice sheath blight fungus Rhizoctonia solani. Molecules, 23(2), 276. https://doi.org/10.3390/MOLECULES23020276

Lai, C. T., Hsieh, Y. H., Wang, Y. H., Chang, K. F., Sun, W. C., Yu, C. L., Lee, T. H., Wang, S. W., & Lin, C. L. (2025). Trichodermin, an endophytic fungal sesquiterpene, suppresses colorectal cancer cell migration and invasion by targeting the PKC-ERK-Sp1-CTSV axis. Phytomedicine, 145, 157063. https://doi.org/10.1016/J.PHYMED.2025.157063

Liu, Y., He, P., He, P., Munir, S., Ahmed, A., Wu, Y., Yang, Y., Lu, J., Wang, J., Yang, J., Pan, X., Tian, Y., & He, Y. (2022). Potential biocontrol efficiency of Trichoderma species against oomycete pathogens. Frontiers in Microbiology, 13, 974024. https://doi.org/10.3389/FMICB.2022.974024

Mao, T., Chen, X., Ding, H., Chen, X., & Jiang, X. (2020). Pepper growth promotion and Fusarium wilt biocontrol by Trichoderma hamatum MHT1134. Biocontrol Science and Technology, 30(11), 1228–1243. https://doi.org/10.1080/09583157.2020.1803212

Mirsam, H., Suriani, Kurniawati, S., Purwanto, O. D., Muis, A., Pakki, S., Tenrirawe, A., Nonci, N., Herawati, Muslimin, & Azrai, M. (2023). In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings. Egyptian Journal of Biological Pest Control, 33(1), 1–14. https://doi.org/10.1186/S41938-023-00729-5

Nielsen, K. F., Gräfenhan, T., Zafari, D., & Thrane, U. (2005). Trichothecene production by Trichoderma brevicompactum. Journal of Agricultural and Food Chemistry, 53(21), 8190–8196. https://doi.org/10.1021/JF051279B

Pedras, M. S. C., & Hossain, M. (2006). Metabolism of crucifer phytoalexins in Sclerotinia sclerotiorum: detoxification of strongly antifungal compounds involves glucosylation. Organic & Biomolecular Chemistry, 4(13), 2581–2590. https://doi.org/10.1039/B604400J

Pedras, M. S. C., & Khan, A. Q. (2000). Biotransformation of the phytoalexin camalexin by the phytopathogen Rhizoctonia solani. Phytochemistry, 53(1), 59–69. https://doi.org/10.1016/S0031-9422(99)00479-3

Pérez-Hernández, A., Serrano-Alonso, Y., Aguilar-Pérez, M. I., Gómez-Uroz, R., & Gómez-Vázquez, J. (2014). Damping-off and root rot of pepper caused by Fusarium oxysporum in Almería Province, Spain. Plant Disease, 98(8), 1159. https://doi.org/10.1094/PDIS-02-14-0212-PDN

Raffa, C. M., & Chiampo, F. (2021). Bioremediation of agricultural soils polluted with pesticides: A Review. Bioengineering, 8(7), 92. https://doi.org/10.3390/BIOENGINEERING8070092

Salvatore, M. M., Masi, M., DellaGreca, M., & Andolfi, A. (2025). The phytopathogenic fungus Macrophomina phaseolina: a potential resource for biosynthesis and biotransformation of bioactive compounds. Phytochemistry Reviews, 1431–1464. https://doi.org/10.1007/S11101-025-10168-9

Sanabria Velázquez, A. D. (2020). Evaluación de aislados de Trichoderma spp. nativos del Paraguay para el control de Colletotrichum spp. causante de la antracnosis en frutilla. Investigación Agraria, 22(1), 53–62. https://doi.org/10.18004/INVESTIG.AGRAR.2020.JUNIO.53-62

Sanabria-Velázquez, A. D., Bittar-Vega, H. K., Montiel, G., Cantero, F., Britos, R., Ortiz, C., & Sarubbi-Orué, H. J. (2025). Biological control of Sclerotium rolfsii Sacc. in Stevia rebaudiana using native isolates of Trichoderma spp. from Paraguay. Agronomía Colombiana, 43(1), e117744. https://doi.org/10.15446/agron.colomb.v43n1.117744

Sanabria-Velázquez, A. D., Pavía, M. M. F., Ayala, L. I., Flores-Giubi, M. E., Romero-Rodríguez, M. C., Sotelo, P. H., & Barúa, J. E. (2023). Characterization of Trichoderma species from agricultural soils of Paraguay. Agronomía Colombiana, 41(3), e111299. https://doi.org/10.15446/agron.colomb.v41n3.111299

Sánchez-Montesinos, B., Diánez, F., Moreno-Gavira, A., Gea, F. J., & Mila Santos, M. (2019). Plant growth promotion and biocontrol of Pythium ultimum by saline tolerant Trichoderma isolates under salinity stress. International Journal of Environmental Research and Public Health 2019, 16 (11). https://doi.org/10.3390/IJERPH16112053

Savary, S., Willocquet, L., Pethybridge, S. J., Esker, P., McRoberts, N., & Nelson, A. (2019). The global burden of pathogens and pests on major food crops. Nature Ecology and Evolution, 3(3), 430–439. https://doi.org/10.1038/S41559-018-0793-Y

Stange, P., Kersting, J., Sivaprakasam Padmanaban, P. B., Schnitzler, J. P., Rosenkranz, M., Karl, T., & Benz, J. P. (2024). The decision for or against mycoparasitic attack by Trichoderma spp. is taken already at a distance in a prey-specific manner and benefits plant-beneficial interactions. Fungal Biology and Biotechnology, 11 (14), 1–23. https://doi.org/10.1186/S40694-024-00183-4

Tijerino, A., Elena Cardoza, R., Moraga, J., Malmierca, M. G., Vicente, F., Aleu, J., Collado, I. G., Gutiérrez, S., Monte, E., & Hermosa, R. (2011). Overexpression of the trichodiene synthase gene tri5 increases trichodermin production and antimicrobial activity in Trichoderma brevicompactum. Fungal Genetics and Biology, 48(3), 285–296. https://doi.org/10.1016/j.fgb.2010.11.012

Tudi, M., Ruan, H. D., Wang, L., Lyu, J., Sadler, R., Connell, D., Chu, C., & Phung, D. T. (2021). Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health, 18(3), 1112. https://doi.org/10.3390/IJERPH18031112

Vinale, F., Marra, R., Scala, F., Ghisalberti, E. L., Lorito, M., & Sivasithamparam, K. (2006). Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology, 43(2), 143–148. https://doi.org/10.1111/J.1472-765X.2006.01939.X

Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., & Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry, 40(1), 1–10. https://doi.org/10.1016/J.SOILBIO.2007.07.002

Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Woo, S. L., Nigro, M., Marra, R., Lombardi, N., Pascale, A., Ruocco, M., Lanzuise, S., Manganiello, G., & Lorito, M. (2014). Trichoderma secondary metabolites active on plants and fungal pathogens. The Open Mycology Journal, 8(1), 127–139. https://doi.org/10.2174/1874437001408010127

Vizcaíno, J. A., Sanz, L., Cardoza, R. E., Monte, E., & Gutiérrez, S. (2005). Detection of putative peptide synthetase genes in Trichoderma species: Application of this method to the cloning of a gene from T. harzianum CECT 2413. FEMS Microbiology Letters, 244(1), 139–148. https://doi.org/10.1016/J.FEMSLE.2005.01.036

Wang, Y., Zeng, L., Wu, J., Jiang, H., & Mei, L. (2022). Diversity and effects of competitive Trichoderma species in Ganoderma lucidum–cultivated soils. Frontiers in Microbiology, 13, 1067822. https://doi.org/10.3389/FMICB.2022.1067822

Xiao, C., Li, L., Liu, Y., Huang, Y., Wang, Y., Wang, J., Bao, G., Sun, G., & Lin, F. (2022). Inhibitory effect and mechanism of Trichoderma taxi and its metabolite on Trichophyton mentagrophyte. Journal of Fungi, 8(10), 1006. https://doi.org/10.3390/JOF8101006

Xu, X., Cheng, J., Zhou, Y., Zhang, C., Ou, X., Su, W., Zhao, J., & Zhu, G. (2013). Synthesis and antifungal activities of trichodermin derivatives as fungicides on rice. Chemistry and Biodiversity, 10(4), 600–611. https://doi.org/10.1002/CBDV.201200135

Yanar, Y., & Miller, S. A. (2003). Resistance of pepper cultivars and accessions of Capsicum spp. to Sclerotinia sclerotiorum. Plant Disease, 87(3), 303–307. https://doi.org/10.1094/PDIS.2003.87.3.303

Zhang, J. L., Tang, W. L., Huang, Q. R., Li, Y. Z., Wei, M. L., Jiang, L. L., Liu, C., Yu, X., Zhu, H. W., Chen, G. Z., & Zhang, X. X. (2021). Trichoderma: a treasure house of structurally diverse secondary metabolites with medicinal importance. Frontiers in Microbiology, 12, 723828. https://doi.org/10.3389/FMICB.2021.723828

Zhang, F., Ge, H., Zhang, F., Guo, N., Wang, Y., Chen, L., Ji, X., & Li, C. (2016). Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean. Plant Physiology and Biochemistry, 100, 64–74. https://doi.org/10.1016/j.plaphy.2015.12.017

Descargas

Publicado

2026-06-30

Cómo citar

Metabolitos secretados por aislados nativos de Trichoderma spp. y su actividad antifúngica frente a Rhizoctonia solani y Sclerotinia sclerotiorum, patógenos del pimientoos del pimiento. (2026). Revista Investigaciones Y Estudios - UNA, 17(1), 21-33. https://revistascientificas.una.py/index.php/rdgic/article/view/5770

Artículos similares

21-30 de 81

También puede Iniciar una búsqueda de similitud avanzada para este artículo.