Good Manufacturing Practices of butcher shops and frequency of Shiga toxin-producing Escherichia coli and Salmonella spp. from ground meat from butcher shops in Asunción, Paraguay

Authors

DOI:

https://doi.org/10.18004/mem.iics/1812-9528/2024.e22122402

Keywords:

Hygienic-sanitary quality, Butcher shops, Hygienic-sanitary quality, Butcher shops, STEC, Salmonella, Salmonella

Abstract

The safety of marketed meat must be guaranteed in the production chain, to avoid foodborne illness. Shiga toxin-producing Escherichia coli (STEC) and Salmonella spp. can be found in the gastrointestinal tract of cattle and contaminate meat for human consumption, potentially causing diseases in humans. This work aimed to evaluate the hygienic-sanitary conditions of 52 butcher shops located in Asunción and detect the frequency of STEC and Salmonella spp. in ground beef samples. Hygienic-sanitary conditions were evaluated by estimating risk, using a categorical classification scale. The detection of STEC and Salmonella spp. was performed by real-time PCR. In the initial evaluation, 33% of the butcher shops were classified as high and moderate risk. STEC non-O157 was detected in 50% (130/258) of the samples and Salmonella spp. in 11% (29/258). Improvement actions were carried out. In the post-intervention stage, no high-risk butcher shops were detected. In the follow-up sampling, 29% (66/237) of positive samples were detected for STEC non-O157 and 7% (16/237) for Salmonella spp. This study allowed specific and detailed recommendations to be made to each butcher shop, which had a significant effect on improving their conditions. This situation highlights the importance of continuing to strengthen multisectoral and multidisciplinary surveillance. It is imperative that establishments dedicated to the sector implement Good Manufacturing Practices (GMP) as a measure to reduce associated risks.

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Published

2024-02-28

How to Cite

Weiler, N., Orrego, M., Álvarez, M., Ortiz, F., Martínez, J., Dure, F., … Leotta, G. (2024). Good Manufacturing Practices of butcher shops and frequency of Shiga toxin-producing Escherichia coli and Salmonella spp. from ground meat from butcher shops in Asunción, Paraguay. Memorias Del Instituto De Investigaciones En Ciencias De La Salud, 22(1). https://doi.org/10.18004/mem.iics/1812-9528/2024.e22122402

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