Plant parasitic nematodes in the spotlight: exploring the pathogenic potential of the genera Helicotylenchus, Scutellonema, and Tylenchorhynchus in soybean in Paraguay
DOI:
https://doi.org/10.57201/IEUNA2313335Keywords:
Scutellonema, Helicotylenchus, Tylenchorhynchus, Soybean, Reproductive Factor.Abstract
Soybean is considered one of the most important crops in the world, and nematodes are equally important globally due to their ability to cause diseases. Phytoparasitic nematodes of the genera Helicotylenchus, Scutellonema, and Tylenchorhynchus have been reported in several soybean production areas. This study aimed to determine the reproductive factor of Helicotylenchus, Scutellonema, and Tylenchorhynchus in soybean crops. Four commercial soybean fields were selected in Itapúa and Canindeyú, which were named commercial plot 1, 2, 3, and 4. Within these fields, 10 composite samples were taken at the beginning of planting (initial population) and at harvest time (final population). The reproductive factor (RF) for each phytoparasitic nematode was evaluated using the Student's t-test, comparing if the RF was equal to or less than one (Ho) or greater than one (Ha). Helicotylenchus nematode was detected in all four plots, but only in commercial plots 3 and 4, the RF was significantly higher than 1 (P-value). The presence of Scutellonema was identified in commercial plots 1, 2, and 4, and the RF was significantly higher than 1 (P-value). Tylenchorhynchus was identified in commercial plots 2 and 3, and in both cases, the RF was significantly higher than 1. Therefore, it was confirmed that the genera Helicotylenchus, Scutellonema, and Tylenchorhynchus reproduce in soybean-associated fields, making them potential pathogens of this crop in Paraguay.
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References
Aboul-Eid, A. H., & Osman, H. A. (1981). Control of Tylenchorhynchus clarus on soybean with systemic
nematicides. Nematologia Mediterranea, 9, 105–107.
https://journals.flvc.org/nemamedi/article/view/85390
Anwar, S. A., & McKenry, M. V. (2010). Incidence and reproduction of Meloidogyne incognita on vegetable crop
genotypes. Pakistan Journal of Zoology, 42, 135–141.
Baida F. C., Stroze C. T., Machado A. C. Z., & Amaro P. M. (2015ª). Ocorrência de Helicotylenchus dihystera em
cultivo de soja no Paraná. Memoria del XXXII Congresso Brasileiro de Nematologia, 15 a 19 de junio de
(pp.89). Londrina, Estado de Parana, Brasil. http://nematologia.com.br/wp-
content/uploads/2015/06/anaiscbn32.pdf
Baida F. C., Stroze C. T., Machado A. C. Z., & Amaro P. M. (2015b) Incidência de Scutellonema brachyurus em
três regiões produtoras de soja no Paraná. Memoria del XXXII Congresso Brasileiro de Nematologia, 15 a
de junio de 2015 (p.88). Londrina, Estado de Parana, Brasil http://nematologia.com.br/wp-
content/uploads/2015/06/anaiscbn32.pdf
Banco Central del Paraguay. (2022). Anexo Estadístico del Informe Económico - BCP.
https://www.bcp.gov.py/anexo-estadistico-del-informe-economico-i36
Cámara Paraguaya de Exportadores y Comercializadores de Cereales y Oleaginosas. (2022). Área de Siembra,
Producción y Rendimiento - soja . http://capeco.org.py/area-de-siembra-produccion-y-rendimiento/
Carosini Ruíz Díaz, A. L., Barrios Leiva, O. S., Nakayama Nakashima, H. D., Garcete Escurra, G., Achinelli Báez,
M., Cabral López, A., Olmedo Barchello, S, Báez Martínez, J. F., & Morínigo Aguayo, J. D. (2020). Impacto
Económico de la Aprobación de la Soja Transgenica en el Paraguaya. San Lorenzo, PY: Universidad
Nacional de Asunción. 132 p.
https://www.conacyt.gov.py/sites/default/files/upload_editores/u294/LIBRO-soja-ECO_UNA-2020-
FEBRERO.pdf
Chen, S. Y., Sheaffer, C. C., Wyse, D. L., Nickel, P., & Kandel, H. (2012). Plant-parasitic Nematode Communities
and Their Associations with Soil Factors in Organically Farmed Fields in Minnesota. Journal of
nematology, 44, 361-369.
Empresa Brasileira de Pesquisa Agropecuária. (2013). Tecnologias de Produção de Soja. Sistemas de Produção.
http://www.cnpso.embrapa.br/download/publicacao/central_2005.pdf.)
Favoreto, L., Meyer, M. C., Dias-Arieira, C. R., Cristina, A., & Machado, Z. (2019). Diagnose e manejo de
Fitonematoides Na Cultura Da Soja. Informe Agropecuário, 40(306), 18–29.
Fourie, H., McDonald, A. H., & Loots, G. C. (2001). Plant-parasitic nematodes in field crops in South Africa.
Nematology, 3(5), 447–454. doi:10.1163/156854101753250773
Food and Agriculture Organization of the United Nations. (2020). Production quantities of Soybeans by country -
Total World. FaoStat. https://www.fao.org/faostat/en/#data/QCL
Fundação Rio Verde. (2014). Levantamento populacional de espécies de nematoides na cultura da soja na
região do médio norte de Mato Grosso, 2013–2014. www.fundacaorioverde.com.br/publicacoes/68.
Gardiano-Link, C. G., de Melo Santana-Gomes, S., Kluge, E. R., Feksa, H. R., da Rosa Kluge, F. T., & Dias-
Arieira, C. R. (2022). Management systems for nematode control in soybean fields in south-central
Paraná, Brazil. Pesquisa Agropecuaria Brasileira, 57, 25-26. https://doi.org/10.1590/S1678-
pab2022.v57.02526
Germani, G. (1981). Pathogenicity of the nematode Scutellonema cavenessi on peanut and soybean. Revue
Nématol, 4(2), 203–208.
Griffiths, B., Neilson, R. & Bengough, A. G. (2003). Soil factors determined nematode community composition in
a two year pot experiment. Nematology, 5(6), 889–897.
Kirsch, V. G., Kulczynski, S. M., Bauer Gomes, C., Calderan Bisognin, A., Gabriel, M., Bellé, C., Lima-Medina, I.,
Kulczynski, S. M., Gomes, C. B., Bisognin, A. C., Gabriel, M., Bellé, C., & Lima-Medina, I. (2016).
Caracterização de espécies de Meloidogyne e de Helicotylenchus associadas à soja no Rio Grande do Sul.
Nematropica, 46(2), 197–208. https://journals.flvc.org/nematropica/article/view/90812
Handoo, Z. A., Palomares-Rius, J. E., Cantalapiedra-Navarrete, C., Liébanas, G., Subbotin, S. A., & Castillo, P.
(2014). Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus
(Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny. Zoological
Journal of the Linnean Society, 172(2), 231–264. https://doi.org/10.1111/zoj.12175
Jenkins, W. R. (1964). A rapid centrifugalotation technique for separating nematodes from soil. Plant Disease
Reporter, 48(9), 692.
Koenning, S. R., & Barker, K. R. (1998). Survey of Heterodera glycines Races and Other Plant-parasitic
Nematodes on Soybean in North Carolina. Journal of Nematology, 30(4), 569.
/pmc/articles/PMC2620330/?report=abstract
Gini-Alvarez et al. Nematodos fitoparásitos en la mira: explorando el potencial patogénico…
Rev. Invest. y Est.- UNA, Volumen 14, Número 1, 2023: x-x pp.x/x
Kraus, H., & Lewis, S. (1979). Scutellonema brachyurum: Host Plants and Pathogenicity on Cotton. Plant
Disease Reporter, 63(8), 688–691.
https://books.google.com.py/books?hl=es&lr=&id=AD4iadwANmYC&oi=fnd&pg=PA688&dq=scutellonem
a+reproductive+factor+in+soybean&ots=E-N46Nm6ne&sig=2qcx-
bKBBa4Cbk4x9s7K_Qf0Fg&redir_esc=y#v=onepage&q&f=false
Lewis, S. A., Drye, C. E., Saunders, J. A., Shipe, E. R., & Halbrendt, J. M. (1993). Plant-parasitic Nematodes on
Soybean in South Carolina. Journal of Nematology, 25(4), 890.
/pmc/articles/PMC2619450/?report=abstract
Lima, F. S. O, Nogueira, S. R, Lima Neto, A. F., Cares, J. E, Furlanetto C., & Dias, T. S. (2009). Ocorrência de
Tubixaba sp. em lavouras comerciais de soja no estado do Tocantins. Tropical Plant Pathology, 34, 207.
Lopes, C. M. L. (2015). Populações de nematoides fitoparasitas em áreas de cultivo de soja, algodão, café e de
vegetação nativa do Cerrado na região Oeste da Bahia. Dissertação de mestrado. Programa de Pós-
graduação em Fitopatologia, Universidade de Brasilia. Brasilia.
López-Nicora, H. D., Soilán-Duarte, L. C., Caballero-Mairesse, G. G., Grabowski-Ocampos, C. J., & Enciso-
Maldonado, G. A. (2021). Manual de nematologia agrícola: bases y procedimentos. Asunción, PY: Grupo
Editorial Atlas. 90 p. https://doi.org/10.53997/DFXA5914
Maceda A., Silva J. F., Dias W. P., Santos J. M., Soares P. L. M, Marcondes, M. C. (2009). Ocorrência de
Scutellonema brachyurus Andrassy em soja no Brasil. Em Memoria de II International Congress of
Tropical Nematology,4 a 9 de junio de 2009 (pp. 347). Maceió, Brasil.
https://nematologia.com.br/files/revnb/33_4.pdf
Machado, A. C. Z., Amaro, P. M., & Da Silva, S. A. (2019a). Two novel potential pathogens for soybean. PLoS
ONE, 14(8), 1–13. https://doi.org/10.1371/journal.pone.0221416
Machado, A. C. Z., da Silva, S. A., & Ferraz, L. C. C. B. (2019b). Métodos em nematologia agrícola. Piracicaba,
Brasil: Sociedade Brasileira de Nematologia. 184 p. https://nematologia.com.br/files/livros/book5.pdf
Machado, A. C. Z. (2014). Current nematode threats to Brazilian agriculture. Current Agricultural Science and
Technology, 20(1), 26–35. https://periodicos.ufpel.edu.br/ojs2/index.php/CAST/article/view/3737
Machado, A. C. Z., Dorigo, O.F., Silva, S.A., Amaro, P.M. (2015a). Parasitismo de Helicotylenchus dihystera nas
culturas da soja e milheto. Memoria del XXXII Congresso Brasileiro de Nematologia, 15 a 19 de junio de
(p.89). Londrina, Estado de Parana, Brasil. https://nematologia.com.br/files/anais/2015/Anais - 32
CBN - 2015.pdf
Machado, A. C. Z., Dorigo, O. F., Silva, S. A., & Amaro, P.M. (2015b). Parasitismo de Scutellonema brachyurus
na cultura da soja. Memoria del XXXII Congresso Brasileiro de Nematologia, 15 a 19 de junio de 2015
(p.162). Londrina, Estado de Parana, Brasil. https://nematologia.com.br/files/anais/2015/Anais - 32 CBN -
Morínigo Aguayo, J., Achinelli Báez, M., & Barrios Leiva, O. (2018). La soja en el Paraguay. Una aproximación a
la cuantificación económica. Periodo 1994-2016. Población y Desarrollo, 24(46), 24–36.
https://doi.org/10.18004/pdfce/2076-054x/2018.024(46).024-036
Neto, L. J. T. (2019). Correlação espacial da resistência do solo à penetração e densidade populacional de
nematoides na cultura da soja. (Tesis de maestria). Instituto Federal Goiano.
https://sistemas.ifgoiano.edu.br/sgcursos/uploads/anexos_1/2019-12-03-12-26-
Lauro%20Joaquim%20Tiago%20Neto.pdf
Niblack, T. L. (1992). Pratylenchus, Paratylenchus, Helicotylenchus, and other nematodes on soybean in
missouri. Journal of Nematology, 24(4), 738–744.
Norton, D. C. (1977). Helicotylenchus pseudorobustus as a pathogen on corn, and its densities on corn and
soybean. Iowa State Journal of Research, (51), 279–285.
Pedrozo, L. (2008). Nematodos del cultivo de soja en Paraguay. Caacupé, PY: MAG-DIA-IAN/INBIO. 32 p.
https://www.inbio.org.py/informes/publicaciones/Nematodos-del-cultivo-de-soja-en-Paraguay.pdf
Oostenbrink, M. (1966). Major characteristics of the relation between nematodes and plants. Meded Landbouw,
Wageninqen, (66), 446.
RStudio Team. (2018). RStudio: Integrated Development for R (1.2.1335). RStudio, Inc.
Silva, F. C. (2007). Levantamento de fitonematoides nas culturas de soja e milho no município de Jataí–GO.
(Tesis de maestria). Universidade Federal de Uberlândia.
https://repositorio.ufu.br/bitstream/123456789/12254/1/FGSilvaDISPRT.pdf
Soares, P. L. M., & Santos, J. (2009). Resistance of soybean cultivars to Scutellonema brachyurus. En XXVIII
Congresso Brasileiro de Nematologia, Memoria de II International Congress of Tropical Nematology,4 a 9
de junio de 2009 (pp. 318). https://nematologia.com.br/files/revnb/33_4.pdf
Taylor, D. P. (1960). Host range study of the spiral nematode. Helicotylenchus microlobus. Plant Disease
Reporter 44, 747-750.
Venditti, M., & Noel, G. (1994). Comparative host suitability of selected crop species for Tylenchorhynchus
zambiensis. Nematropica, 25(1), 15-25. https://journals.flvc.org/nematropica/article/view/64116
Yan, G. P., Plaisance, A., Huang, D., & Handoo, Z. A. (2018). First report of a new stunt nematode
Tylenchorhynchus sp. from a soybean field in North Dakota. Plant Disease, 102(2), 453.
https://doi.org/10.1094/PDIS-05-17-0616-PDN
Yan, G., Plaisance, A., Huang, D., & A. Handoo, Z. (2017). First Report of the Spiral Nematode Helicotylenchus
microlobus Infecting Soybean in North Dakota. Journal of Nematology, 49(1), 1–1.
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Copyright (c) 2023 Horacio Lopez-Nicora; Alejandro Gini-Alvarez, Fernando Lugo (Autor/a); Gabriela Caballero-Mairesse, Guillermo Enciso-Maldonado, Diego Bonussi, Fernando Lombardo, Víctor Lezcano , Federico Barreto, Linzy Espinoza
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