Detecção e caracterização molecular de Anaplasma marginale no DNA de amostras de sangue de bovinos do Paraguai
Palavras-chave:
ADN, Anaplasma marginale, cPCR, bovino, sangueResumo
Anaplasma marginale é uma bactéria intraeritrocitária responsável pela doença conhecida como anaplasmose bovina, que tem um grande impacto na produção pecuária em regiões tropicais e subtropicais. A importância do uso da PCR convencional (cPCR) reside na detecção precoce de infecções persistentes. Além disso, outros métodos de estudo podem apresentar limitações na hora de realizar o diagnóstico. A região do gene Msp5 é específica para a detecção de infecções persistentes. O presente trabalho foi realizado utilizando 86 amostras de DNA bovino (sangue total) através da técnica de cPCR para a amplificação do gene Msp5. 35% (30/86) das amostras foram positivas para Anaplasma marginale. Das amostras positivas, três foram sequenciadas. A análise determinou que o A. marginale obteve um nível de identidade de 99-100%. A construção da árvore filogenética demonstrou que as amostras obtidas estavam intimamente relacionadas a outras sequências anteriormente relatadas em países da Ásia e da África. Este estudo evidencia a prevalência da doença no gado paraguaio. Além disso, destaca-se como a primeira evidência molecular de A. marginale no Paraguai, pelo que se recomenda ampliar os estudos sobre sua patogenicidade, mecanismo de transmissão e distribuição geográfica.
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Copyright (c) 2025 Olga Lorena Nuñez Yegros, Maria de Lourdes Narvaez Quiñonez, Alba Maria Araujo Oviedo, Guillermo Giménez Bareiro, Ingrid Paloma Florentín Ibarra, Lino César Ramírez Ayala
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