Our day-to-day at Memorias del IICS

A glimpse into our day-to-day at the Memorias del IICS office

Leer más

About

Memorias del Instituto de Investigaciones en Ciencias de la Salud (Mem. Inst. Investig. Cienc. Salud) is the official publication of the Institute of Research in Health Sciences (IICS) of the National University of Asunción (UNA), Paraguay. It publishes original scientific contributions in clinical medicine, biomedical research, microbiology, molecular biology, dentistry, nursing, nutrition, public health, health biotechnology, genetics, biochemistry, and related disciplines. Founded in 1983, the journal adopted its current name in 2002 and has been published exclusively online since 2015, incorporating XML article dissemination as of 2024. Since 2023, it operates under a continuous publication model. The journal accepts manuscripts in Spanish and English as original research articles, reviews, case studies, and brief communications. All content is available in open access, free of charge for authors and readers, under the Creative Commons Attribution 4.0 International License, in accordance with the Budapest Open Access Initiative (BOAI). It is indexed in BVS, SciELO Paraguay, HINARI, LILACS, DOAJ, Latindex, MIAR, Dialnet, CiteFactor, Google Scholar, LivRe, BASE, EBSCO, and Web of Science – SciELO Citation Index. The journal is funded by IICS-UNA, supports the OAI-PMH protocol for metadata harvesting, and uses the LOCKSS system for digital preservation of its contents.

 
Leer más

Peer Review Process

Manuscripts submitted to Memorias del Instituto de Investigaciones en Ciencias de la Salud undergo a double-blind peer review process, in which the identities of both authors and reviewers remain anonymous throughout the evaluation. Each manuscript is assessed by a minimum of two external reviewers with expertise in the relevant subject area, who are given a period of 30 days to submit their evaluation. Reviewers assess the originality, methodological rigor, scientific relevance, and clarity of presentation of each work. Based on the reviewers' reports, the Editorial Committee decides whether to accept, request revisions, or reject the manuscript, and communicates the decision to the authors as promptly as possible. This process ensures impartiality, transparency, and the scientific quality of published articles.

Leer más

Call for Papers

The Institute of Research in Health Sciences (IICS) of the National University of Asunción (UNA) invites researchers, academics, and health professionals to submit their manuscripts for consideration in the 2026 Volume of Memorias del Instituto de Investigaciones en Ciencias de la Salud (Mem. Inst. Investig. Cienc. Salud). The journal accepts original and unpublished contributions in Spanish as original research articles, review articles, case studies, and brief communications, in the areas of clinical medicine, biomedical research, microbiology, molecular biology, dentistry, nursing, nutrition, public health, health biotechnology, genetics, biochemistry, and related disciplines. The call for submissions is open and ongoing, and manuscripts may be submitted at any time of the year through the journal's online submission system. All received manuscripts undergo double-blind peer review. The journal is open access, with no charges for authors or readers. For further information on submission guidelines, please refer to the author instructions available on the journal's website.

Leer más

Frecuencia de los patrones de anticuerpos anti-nucleares en pacientes con sospecha clínica de LES

Authors

  • MM Carpinelli Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Asunción (UNA). Paraguay
  • Vivian Giménez Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Asunción (UNA). Paraguay Author
  • Lorena Ferreira Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción (UNA) Author
  • Cristina Rovira Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción (UNA) Author
  • Estela Picaguá Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Asunción (UNA). Paraguay Author
  • Enrique Granados Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Asunción (UNA). Paraguay Author

Keywords:

anticuerpos antinucleares, patrones de tinción, células HEp-2

Abstract

Las enfermedades autoinmunes son alteraciones de los mecanismos de tolerancia inmunológica, con producción de anticuerpos contra antígenos propios, como los anticuerpos antinucleares (ANA), que detectados por inmunofluorescencia indirecta, permiten reconocer varios patrones nucleares y citoplasmáticos, como en el Lupus Eritematoso Sistémico (LES). El objetivo del estudio fue describir los patrones de tinción de ANA en sueros de pacientes con sospecha clínica de LES, que concurrieron al Instituto de Investigaciones en Ciencias de la Salud, durante el año 2008. En este estudio observacional descriptivo, se analizaron 150 sueros positivos para ANA, codificados, respetando la confidencialidad de los mismos. Para la determinación de ANA se utilizaron láminas con células HEp-2 (BIO-RAD-USA), y el conjugado fue anti human IgG de Sigma (USA); la técnica fue inmunofluorescencia indirecta. Del total de 150 pacientes estudiados, 134 (89%) fueron mujeres y 16 (11%) hombres, cuyas edades estaban comprendidas entre 11 y 87 años. De los sueros positivos analizados para ANA, 44 (29%) correspondieron al patrón periférico, 18(12%) puntillado grueso, 39(26%) puntillado fino, 42(28%) homogéneo, 5(3%) nucleolar y 2(1.3%) citoplásmico puntillado fino. En este estudio se encontró en mayor porcentaje el patrón periférico, seguido del  homogéneo y puntillado fino, los cuales se hallan asociados a pacientes con LES, indicando un pronóstico poco favorable para estos pacientes.  

Downloads

Download data is not yet available.

References

Phan TG, Ng WW, Bird D, Smithers K, Wong V, Gallagher K, et a. High-Quality, Cost Effective Strategy for detección of Autoantibodies to extractable Nuclear Antigens. Clin Diag Lab Immunol 2001; 8 (3) 471-74.

Kern P, Kron M, Huesche R. Measurement of Antinuclear Antibodies: Assesment of different test Systems. Microbiology Jan 2000; vol 7 nº 1: p. 72-8.

Damoiseaux JG, Tervaert JW . From ANA to ENA: How to proceed?. Autoinmun Rev 2006; 5 (1): 10-7.

James K, Carpenter AB, Cook L, Marchand R, Nakamura RM. Development of the Antinuclear and Anticytoplasmic Antibody Consensus Panel by the Association of Medical Laboratory Inmunologists. Clin Diagn Lab Immunol. 2000 May;7(3):436-43.

Nesher G, Margalit R, Ashkenazi YJ. Antinuclear Envelope Antibodies: Clinical Associations. Semin Arthritis Rheum. 2001 Apr;30(5):313-20

Gniewek RA, Stites DP, McHugh TM, Hilton JF, Nakagawa M. Comparision of Antinuclear Antibody Testing Methods; Inmunofluorescence Assay versus enzymeinmunoassay. Clinical and Diagnostic Laboratory Inmunology Mar 1997; 4(2): 185-88.

Kiuttu J, Hartikainen AL, Makitalo R, Ruuska P. The outcome of pregnancy in antinuclear antibody-positive women. Gynecol Obstet Invest 1994; 37: 160-63.

Sontheimer RD, McCauliffe DP, Zappi E, Targoff I. Antinuclear antibodies: clinical correlations and

biologic significance. Adv Dermatol 1991; 7: 3.

Fernández-Madrid F, Mattioli M. Antinuclear antibodies (ANA): immunologic and clinical significance. Semin ArthrRheum 1976; VI: 83-124.

Tan EM, Feltkamp TEW, Smolen JS, Butcher B, Dawkins R, Fritzler MJ et al. Range of antinuclear antibodies in ‘healthy’ individuals. Arthritis Rheum. 1997; 40:1601-11

Bernstein RM, Steigerwald JC, Tan EM. Association of antinuclear and antinuclear antibodies in progressive systemic sclerosis. Clin Exp Immunol 1982; 48: 43-51.

Hahn BH. Antibodies to DNA. N Engl J Med 1998; 338: 1359-68.

Suarez-Almazor ME, Gonzalez-Lopez L, Gamez-Nava JI, Belseck E, Kendall CJ, Davis P. Utilization and predictive value of laboratory tests in patients referred to rheumatologists by primary care physicians. J Rheumatol. 1998;25:1980- 5.

Ulvestad E, Kanestrom A, Madland TM, Thomassen E, Haga HJ, Vollset SE. Evaluation of diagnostic test for antinuclear antibodies in rheumatological practice. Scand J Inmunol 52(3):309-5.

Nishioka M, Morshed SA, Parveen S, Ming C. Heterogenity of antinuclear antibodies in autoimune

liver diseases. In: Krawitt EI, Wiesner RH, Nishioka M, editores. Autoimune liver diseases, 2nd

edition. Amsterdam:ELSEVIER;1998.p .179-209

Bizzaro N, Tozzoli R, Tonutti E, Piazza A, Manoni F, Ghirandello A et al. Variability between methods to determine ANA, anti-dsDNA and anti-ENA autoantibodies: a collaborative study with the biomedical industry. J Inmunol Methods 1998; 219(1-2): 99-107.

Downloads

Published

2010-12-01

Issue

Vol. 8 No. 2 (2010): Julio-Diciembre

Section

Original Articles

How to Cite

Carpinelli, M. ., Giménez, V. ., Ferreira, L., Rovira, C., Picaguá, E., & Granados, E. (2010). Frecuencia de los patrones de anticuerpos anti-nucleares en pacientes con sospecha clínica de LES . Memorias Del Instituto De Investigaciones En Ciencias De La Salud, 8(2), 27-33. https://revistascientificas.una.py/index.php/RIIC/article/view/1668