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Memorias del Instituto de Investigaciones en Ciencias de la Salud (Mem. Inst. Investig. Cienc. Salud) is the official publication of the Institute of Research in Health Sciences (IICS) of the National University of Asunción (UNA), Paraguay. It publishes original scientific contributions in clinical medicine, biomedical research, microbiology, molecular biology, dentistry, nursing, nutrition, public health, health biotechnology, genetics, biochemistry, and related disciplines. Founded in 1983, the journal adopted its current name in 2002 and has been published exclusively online since 2015, incorporating XML article dissemination as of 2024. Since 2023, it operates under a continuous publication model. The journal accepts manuscripts in Spanish and English as original research articles, reviews, case studies, and brief communications. All content is available in open access, free of charge for authors and readers, under the Creative Commons Attribution 4.0 International License, in accordance with the Budapest Open Access Initiative (BOAI). It is indexed in BVS, SciELO Paraguay, HINARI, LILACS, DOAJ, Latindex, MIAR, Dialnet, CiteFactor, Google Scholar, LivRe, BASE, EBSCO, and Web of Science – SciELO Citation Index. The journal is funded by IICS-UNA, supports the OAI-PMH protocol for metadata harvesting, and uses the LOCKSS system for digital preservation of its contents.

 
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First whole genome sequencing of Escherichia coli carrying mcr-1 from pig in Argentina

Authors

  • Juan Leandro Pellegrini Universidad Nacional del Nordeste (UNNE), Instituto de Medicina Regional. Resistencia, Chaco, Argentina https://orcid.org/0000-0003-1546-3940
  • Melina Lorenzini-Campos 1)Universidad Nacional del Nordeste (UNNE), Instituto de Medicina Regional. Resistencia, Chaco, Argentina. 2) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Buenos Aires, Argentina https://orcid.org/0009-0003-9092-3879
  • Raúl Horacio Lucero Universidad Nacional del Nordeste (UNNE), Instituto de Medicina Regional. Resistencia, Chaco, Argentina https://orcid.org/0000-0002-8781-8512
  • Ariel Amadio Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Buenos Aires, Argentina. Instituto de Investigación de la Cadena Láctea (IDICAL-INTA-CONICET). Rafaela, Santa Fe, Argentina https://orcid.org/0000-0002-1147-4485
  • Liliana Silvina Lösch Universidad Nacional del Nordeste (UNNE), Instituto de Medicina Regional. Resistencia, Chaco, Argentina https://orcid.org/0000-0002-5096-5578
  • José Alejandro Di Conza Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Buenos Aires, Argentina. Universidad de Buenos Aires (UBA), Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina https://orcid.org/0000-0003-0520-1744
  • Luis Antonio Merino Universidad Nacional del Nordeste (UNNE), Instituto de Medicina Regional. Resistencia, Chaco, Argentina https://orcid.org/0000-0002-7525-3921

DOI:

https://doi.org/10.18004/mem.iics/1812-9528/2024.e22162403

Keywords:

colistin, antibiotic resistance, one health, next-generation sequencing, (Source: MeSH)

Abstract

The objective of this study is to communicate the findings of the first whole genome sequencing of a colistin-resistant Escherichia coli isolate harboring mcr-1 gene obtained from a pig in Argentina. Genomic DNA was sequenced using the MinION Oxford Nanopore platform. The libraries were prepared using a SQK-RBK110-96 protocol. The sequencing process was conducted on a MinION Mk1C MIN 101-C, utilizing a FLO-MIN106 flow cell. The quality of the reads was evaluated using NanoPlot. De novo assembly was conducted using Canu 1.6 and the quality of contigs was evaluated using QUAST. Annotation was performed using Prokka. The CBC20 strain exhibited a colistin MIC of 4 µg/mL. The genome size was 5178653 bp with a GC content of 50,31%. The N50 value was 133,250, while the L50 value was 21. A total of 11,620 genes, 11,518 coding sequences, 77 transfer RNAs and 24 ribosomal RNAs were identified. A serotype O9:H37 with sequence type ST-297 was observed. A total of seven antimicrobial resistance genes were identified, including mcr-1.5, blaTEM-1B, blaEC-18, blaTEM-70, aph(3')-Ia, mph(A) and sul3. The presence of punctual mutations was observed in the genes encoding the proteins GyrA (S83L, D87N) and ParC (S80I). Five distinct plasmid replicon types were identified, including IncFII, IncY, IncFIB, IncX1 and Col440II. Our findings may assist in the comprehension of the mechanisms of antimicrobial resistance, genomic epidemiology and dissemination of mcr-1 gene among animals and environment, which could potentially impact human health.

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References

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Published

2024-07-31

Issue

Vol. 22 No. 1 (2024)

Section

Short communications

How to Cite

Pellegrini, J. L., Lorenzini-Campos, M., Lucero, R. H., Amadio, A., Lösch, L. S., Di Conza, J. A., & Merino, L. A. (2024). First whole genome sequencing of Escherichia coli carrying mcr-1 from pig in Argentina. Memorias Del Instituto De Investigaciones En Ciencias De La Salud, 22(1). https://doi.org/10.18004/mem.iics/1812-9528/2024.e22162403

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