Evaluación del crecimiento y esporulación in vitro de Cercospora sp. y Septoria sp. en diferentes medios de cultivo
DOI:
https://doi.org/10.18004/rcfacen.2025.16.1.019Keywords:
Phytopathogenic fungi, growth, in vitro, culture medium, soybeanAbstract
This study aimed to evaluate the in vitro mycelial growth and sporulation capacity of the
phytopathogenic fungi Cercospora sp. and Septoria sp. (causal agents of Soybean Leaf Blight and Brown Spot,
respectively) in different culture media: Leaf Decoction Agar (LDA), Grain Decoction Agar (GDA), and Potato
Dextrose Agar (PDA). Mycelial growth was measured every three days for 15 days of incubation, and spore
production was quantified at the end of the experiment using a Neubauer chamber. Significant differences in
vegetative growth among the media were detected. For Cercospora sp., the PDA medium exhibited the highest
growth from day 6 to day 15, reaching an average of 21.44 mm, significantly higher than GDA and LDA. In
contrast, for Septoria sp., the GDA medium was the most effective, reaching an average growth of 8.84 mm
by day 15, while PDA showed the lowest growth (3.65 mm). This study represents the first report on the use
of the GDA medium for studying the in vitro growth of Septoria sp. The results demonstrated that none of the
tested media induced sporulation under the evaluated conditions, with only sterile mycelial growth observed.
It is concluded that, although the evaluated media supported mycelial growth, it is necessary to explore new
environmental conditions and alternative media to optimize sporulation, given the relevance of these species in
the epidemiology and management of soybean diseases.
References
Alloatti, J., Li, S., Chen, P., Jaureguy, L., Smith, S. F., Florez-Palacios, L. & Rupe, J. (2015). Screening a diverse soybean germplasm collection for reaction to purple seed stain caused by Cercospora kikuchii. Plant Disease, 99(8): 1140– 1146. <https://doi.org/10.1094/PDIS-09- 14-0878-RE>.
Arrua, A. A., Lopez-Nicora, H. D., Fernández Ríos, D., Grabowski, C. J., Cazal-Martínez, C. C. & Kohli, M. M. (2021). El impacto de las enfermedades fúngicas en el cultivo de soja (Glycine max) en Paraguay ¿es posible estimar las pérdidas. Investigaciones y Estudios - Universidad Nacional de Asunción, 12(1): 34–46. .
Beckman, P. M. & Payne, G. A. (1983). Cultural techniques and conditions influencing growth and sporulation of Cercospora zeae-maydis and lesion development in corn. Phytopathology, 73(2): 286–289. .
Bertagnolli, P. F., Porto, M. D. D. M. & Reis, E. M. (1986). The influence of culture media on the sporulation of Septoria glycines Hemmi, causal agent of soybean brown spot. Pesquisa Agropecuária Brasileira, 21(6): 615–618. <https://doi.org/10.1590/ S1678-3921.pab1986.v21.14846>.
Brunelli, K. R., Fazza, A. C., Athayde Sobrinho, C. & Camargo, L. E. A. (2006). Efeito do meio de cultura e do regime de luz na esporulação de Cercospora zeae-maydis. Summa Phytopathologica, 32: 92–94.
Chand, R., Kumar, P., Singh, V. & Pal, C. (2013). Technique for spore production in Cercospora canescens. Indian Phytopathology, 66(2): 159–163. .
Crous, P. W., Verkley, G. J. M., Groenewald, J. Z. & Houbraken, J. (Eds.) (2019). Fungal Biodiversity. Utrecht: Westerdijk Fungal Biodiversity Institute. Westerdijk Laboratory Manual Series, 1: 425 pp.
Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M. & Robledo, C. W. (2020). InfoStat versión 2020. Córdoba: Centro de Transferencia InfoStat de la Universidad Nacional de Córdoba. [Consulted: 4.vii.2025]. .
El-Gholl, N. E., Alfieri Jr, S. A., Ridings, W. H. & Schoulties, C. L. (1982). Growth and sporulation in vitro of Cercospora apii, Cercospora arachidicola, Cercospora kikuchii and other species of Cercospora. Canadian Journal of Botany, 60(6): 862– 868.
Kashiwa, T., Lavilla, M. A., Paleo, A. D., Ivancovich, A. J. G. & Yamanaka, N. (2021). The use of detached leaf inoculation for selecting Cercospora kikuchii resistance in soybean genotypes. PhytoFrontiers™, 1(4): 250–257. <https://doi.org/10.1094/ PHYTOFR-01-21-0002-TA>.
Quaedvlieg, W., Verkley, G., Shin, H. D., Barreto, R., Alfenas, A., Swart, W. J., Groenewald, J. Z. & Crous, P. W. (2013). Sizing up Septoria. Studies in Mycology, 75: 307– 390. <https://doi.org/10.3114/sim0017>.
Saidi, A., Eslahi, M. R. & Safaie, N. (2012). Efficiency of Septoria tritici sporulation on different culture media. Trakia Journal of Sciences, 10(3): 15–8.
Sautua, F. J., Pizá, M. C. P., Scandiani, M. M. & Carmona, M. A. (2024). Cercospora leaf blight and purple seed stain of soybean: A permanent challenge. Plant Pathology, 73(8): 1981–2004. <https://doi.org/10.1111/ ppa.13956>.
SCABUSA. (2021). US Wheat and Barley Scab Iniciative. Fargo: U.S. Wheat and Barley Scab Initiative. [Consulted: 1.vii.2025]. <https://scabusa.org/>.
Silva, M. G. D., Pozza, E. A., Lima, C. V. R. V. D. & Fernandes, T. J. (2016). Temperature and light intensity interaction on Cercospora coffeicola sporulation and conidia germination. Ciência e Agrotecnologia, 40: 198–204. <https://doi.org/10.1590/1413- 70542016402025915>.
Uppala, S. S., Zhou, X. G., Liu, B. & Wu, M. (2019). Plant-based culture media for improved growth and sporulation of Cercospora janseana. Plant Disease, 103(3): 504–508. <https://doi.org/10.1094/ PDIS-05-18-0814-RE>.
Vathakos, M. G. & Walters, H. J. (1979). Production of conidia by Cercospora kikuchii in culture. Phytopathology, 69(8): 832–833. .
Yeh, C. C. & Sinclair, J. B. (1980). Sporulation and variation in size of conidia and conidiophores among five isolates of Cercospora kikuchii. Plant Disease, 64(4): 373–374. <https://doi.org/10.1094/PD-64- 373>.
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