Experimental liver transplantation in pigs. Application of surgical protocols

Abstract

This research project was conducted between October 2004 and January 2005 to consolidate a multidisciplinary liver transplantation team and contribute to finding a solution for end-stage liver failure. Protocols for organ procurement, back-table surgery, hepatectomy, implantation, and anesthesia were applied in accordance with bioethical principles for the management of experimental animals. Eight Landrace, Large White, or crossbred pigs with a mean weight of 31.25 kg were used. Three animals served as liver and blood donors, three as recipients, and two as blood donors. Mean organ procurement time was 1 hour and 23 minutes, and 3,000 cc of chilled solution was used for organ preservation. Recipient hepatectomy and donor liver implantation required a mean of 3 hours and 12 minutes. Mean cold ischemia time was 3 hours and 7 minutes. Vascular anastomoses were performed according to the established protocols. In the third recipient, biliary reconstruction was completed using a Roux-en-Y choledochojejunostomy. Experimental liver transplantation surgery is feasible in a university setting and should be incorporated into training at the Faculty of Medicine.