First characterization of preantral follicles in the ovaries of adult alpaca
DOI:
https://doi.org/10.18004/mem.iics/1812-9528/2019.017.02.102-106Keywords:
Alpaca, follicle, ovary, histologyAbstract
It is a fact that while even basic reproductive information on alpacas is unavailable, the normal ovarian reserve of this species in comparison to other species is also unidentified. In this study, the ovarian preantral follicles in healthy adult alpacas were characterized in order to establish a general model to in vitro studies. Ten ovaries were collected from five adult alpacas. The ovarian cortex samples were fixed with paraformaldehyde and histological analysis was done. Normal and degenerated follicles percentages were determined. The normal follicles were measured and classified in primordial, transitional, primary and secondary stages. Most of the preantral follicles present in the ovarian cortex of alpacas were primordial and transitional stages; primary (6.10%) and secondary (0.37%) follicles were rarely found. The primary and secondary follicles were larger in diameter when compared with the primordial and transitional follicles. The largest oocyte diameter was recorded in the secondary follicles (P < 0.05). This study serves to establish a biological model for future reproduction studies in Alpacas or as possible biological model for studies of folliculogenesis in humans.
Downloads
References
Bravo PW, Stabenfeldt GH, Lasley BL, Fowler ME. The effect of ovarian follicle size on pituitary and ovarian responses to copulation in domesticated South American camelids. Biol Reprod. 1991 Oct;45(4):553–9.
Sumar JB. Reproduction in female South American domestic camelids. J Reprod Fertil Suppl. 1999;54: 169–78.
Vaughan JL, Tibary A. Reproduction in female South American camelids: A review and clinical observations. Small Rumin Res. 2006 Feb;61(2–3):259–81.
Fortune J. The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Anim Reprod Sci. 2003 Oct;78(3–4):135–63.
van Wezel IL, Rodgers RJ. Morphological characterization of bovine primordial follicles and their environment in vivo. Biol Reprod. 1996 Nov;55(5):1003–11.
Araújo VR, Gomes CM, Deborah M, Modesto G, Báo SN, Viana JR, et al. Effect of Bone Morphogenetic Protein-7 (BMP-7) on in vitro survival of caprine preantral follicles. Pesq Vet Bras. 2010;30(4):305–10.
Cahill LP, Mauléon P. A study of the population of primordial and small follicles in the sheep. J Reprod Fertil. 1981 Jan;61(1):201–6.
Arias P, Feder F-H, Porcel R, Cox J. Estudio histológico comparativo del aparato reproductivo de llama hembra (Lama guanicoe glama). Anat Histol Embryol. 1992 Dec;21(4):314–23.
Amorim CA, Rondina D, Rodrigues APR, Costa SHF, Gonçalves PBD, de Figueiredo JR, et al. Isolated ovine primordial follicles cryopreserved in different concentrations of ethylene
glycol. Theriogenology. 2003 Sep;60(4):735–42.
Figueiredo JR, Hulshof SC., Van den Hurk R, Ectors FJ, Fontes RS, Nusgens B, et al. Development of a combined new mechanical and enzymatic method for the isolation of intact preantral follicles from fetal, calf and adult bovine ovaries. Theriogenology. 1993 Oct;40(4):789–99.
Silva-Santos KC, Santos GMG, Siloto LS, Hertel MF, Andrade ER, Rubin MIB, et al. Estimate of the population of preantral follicles in the ovaries of Bos taurus indicus and Bos taurus taurus cattle. Theriogenology. 2011 Oct;76(6):1051–7.
Magalhães-Padilha DM, Andrade PM, Sales ET, Araujo VR, Lima IMT, Castro S V, et al. Effect of sequential medium on in vitro culture of goat ovarian cortical tissue. Anim Reprod Sci. 2012 Jun;132(3–4):159–68.
