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Memorias del Instituto de Investigaciones en Ciencias de la Salud (Mem. Inst. Investig. Cienc. Salud) is the official publication of the Institute of Research in Health Sciences (IICS) of the National University of Asunción (UNA), Paraguay. It publishes original scientific contributions in clinical medicine, biomedical research, microbiology, molecular biology, dentistry, nursing, nutrition, public health, health biotechnology, genetics, biochemistry, and related disciplines. Founded in 1983, the journal adopted its current name in 2002 and has been published exclusively online since 2015, incorporating XML article dissemination as of 2024. Since 2023, it operates under a continuous publication model. The journal accepts manuscripts in Spanish and English as original research articles, reviews, case studies, and brief communications. All content is available in open access, free of charge for authors and readers, under the Creative Commons Attribution 4.0 International License, in accordance with the Budapest Open Access Initiative (BOAI). It is indexed in BVS, SciELO Paraguay, HINARI, LILACS, DOAJ, Latindex, MIAR, Dialnet, CiteFactor, Google Scholar, LivRe, BASE, EBSCO, and Web of Science – SciELO Citation Index. The journal is funded by IICS-UNA, supports the OAI-PMH protocol for metadata harvesting, and uses the LOCKSS system for digital preservation of its contents.

 
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Manuscripts submitted to Memorias del Instituto de Investigaciones en Ciencias de la Salud undergo a double-blind peer review process, in which the identities of both authors and reviewers remain anonymous throughout the evaluation. Each manuscript is assessed by a minimum of two external reviewers with expertise in the relevant subject area, who are given a period of 30 days to submit their evaluation. Reviewers assess the originality, methodological rigor, scientific relevance, and clarity of presentation of each work. Based on the reviewers' reports, the Editorial Committee decides whether to accept, request revisions, or reject the manuscript, and communicates the decision to the authors as promptly as possible. This process ensures impartiality, transparency, and the scientific quality of published articles.

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The Institute of Research in Health Sciences (IICS) of the National University of Asunción (UNA) invites researchers, academics, and health professionals to submit their manuscripts for consideration in the 2026 Volume of Memorias del Instituto de Investigaciones en Ciencias de la Salud (Mem. Inst. Investig. Cienc. Salud). The journal accepts original and unpublished contributions in Spanish as original research articles, review articles, case studies, and brief communications, in the areas of clinical medicine, biomedical research, microbiology, molecular biology, dentistry, nursing, nutrition, public health, health biotechnology, genetics, biochemistry, and related disciplines. The call for submissions is open and ongoing, and manuscripts may be submitted at any time of the year through the journal's online submission system. All received manuscripts undergo double-blind peer review. The journal is open access, with no charges for authors or readers. For further information on submission guidelines, please refer to the author instructions available on the journal's website.

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Identificación molecular del sexo en 9 especies de aves del Centro de Investigación en Animales Silvestres de la hidroeléctrica de ITAIPU, lado paraguayo

Authors

  • Florencia del Puerto
  • Diana Pésole Author
  • Santiago Molina Author
  • Karen Vera Author
  • Marcela Arias Author
  • Javier Sosa Author
  • María Luisa Ortiz Author
  • José Fernández Author
  • Anastacio Garay Author

Keywords:

Sexado, aves, 2550F/2718R, P2/P8, Paraguay

Abstract

La importancia de determinar el sexo en especies monomórficas que viven en cautiverio conlleva a estrategias de apareamiento a fin de poder incentivar la reproducción. Existen métodos no moleculares de reconocimiento pero son factibles en edad adulta y mucho más difícil cuando son polluelos. Mediante la técnica molecular de PCR, se amplificó el gen de la Cromo Helicasa de Unión al ADN empleando los cebadores, 2550F/2718R. Por este método molecular se consiguió determinar el sexo en 19 de 23 especies de las cuales 3 son ejemplares de: Amazona aestiva, 1 de Pipile grayi, 1 de Bubo virginianus, 4 de Ara chloropterus, 6 de Crax fasciolata, 2 de Caracara plancus, 3 de Chauna torquata, 1 de Cariama cristata y 2 de Cairina moschata del Centro de Investigación de Animales Silvestres de ITAIPU binacional, del lado paraguayo. Nuestros resultados sugieren que el par de cebadores 2550F/2718R podría ser de utilidad para determinar el sexo en las especies estudiadas en el país.

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References

Dubiec A, Zagalska-Neubauer M. Molecular techniques for sex identification in birds. Biological Lett 2006; 43(1): 3-12.

Griffiths R, Daan S, Dijkstra C. 1996. Sex identification in birds using two CHD genes. Proceedings of the Royal Society B 263:1251–6.

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Ellegren H. First gene on the avian W chromosome (CHD) provides a tag for universal sexing on non-ratite birds. Proceedings of the Royal Society B 1996; 263:1635–41.

Bosnjak J, Stevanov-Pavlovic M, Vucicevic M, Stevanovic J, Simeunovic P, Resanovic R, et al. Feasibility of Non-Invasive Molecular Method for Sexing of Parrots. Pakistan Journal of Zoology 2013; 45(3):715-20.

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Campbell T. Hematology. In: Ritchie B, Harrison G, Harrison L. Avian Medicine: Principles and application. Wingers Publishing Inc 1994: 176-98.

Rubio JM, Benito A, Berzosa PJ, Roche J, Puente S, Subirats M, et al. Usefulness of seminested multiplex PCR in surveillance of imported malaria in Spain. Journal of Clinical Microbiology. 1999; 37(10):3260-4.

Fridolfsson A, Ellegren H. A simple and universal method for molecular sexing of nonratite birds. Journal of Avian Biology 1999; 30:116–21.

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Published

2017-12-01

Issue

Vol. 15 No. 3 (2017): Setiembre-Diciembre

Section

Short communications

How to Cite

del Puerto, F., Pésole, D., Molina, S., Vera, K., Arias, M., Sosa, J., Ortiz, M. L., Fernández, J., & Garay, A. (2017). Identificación molecular del sexo en 9 especies de aves del Centro de Investigación en Animales Silvestres de la hidroeléctrica de ITAIPU, lado paraguayo. Memorias Del Instituto De Investigaciones En Ciencias De La Salud, 15(3). https://revistascientificas.una.py/index.php/RIIC/article/view/1939