Keywords
Acinetobacter
Pseudomonas
Drug Resistance
Microbial
Carbapenemase
Urine culture
How to Cite
Abstract
Background: Antibiotic resistance is currently a serious problem around the world. The aim of this study was to determine the prevalence of carbapenemase-producing bacteria in urine cultures and their resistance mechanisms.
Methods: A retrospective cross-sectional study was performed, which included 10.959 urine cultures from June 2017 to June 2018 at the Central Laboratory of the Hospital de Clínicas. The identification of bacterial species was performed with Vitek 2-C®. For the in vitro sensitivity study, the minimum inhibitory concentrations (MIC) were determined according to the Clinical and Laboratory Standards Institute guidelines.
Results: 333 isolates of k. pneumoniae, P. aeruginosa, and Acinetobacter spp were obtained in a period of 13 months, of which 116 (34.83%) were resistant to carbapenems; 16 isolates (29.09%) of K. pneumoniae had the KPC mechanism, 24 (43.64%) MBL and 15 (27.27%) other mechanisms. 6 (13.33%) P. aeruginosa were MBL type, and 39 (86.67%) were found with other mechanisms. For Acinetobacter spp., 1 (6.25%) was MBL type and 15 (93.75%) had other resistant mechanisms. The average age of patients with KPC and MBL strains was 49,45 ± 26,5 years, 66% (31/47) were male, and 34% (16/47) female. The greatest number of resistant strains were from the adult emergency room with 24,14% (28/116). Other bacterial species were not considered.
Conclusion: Early detection of resistant strains and their resistance mechanisms is essential to target a better therapy and patient management. A greater commitment and a better strategy are required to prevent the spread of these infections that threaten the hospital environment.
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